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PPE Donning & Doffing Sequence

Personal protective equipment (PPE) follows two opposite sequences. The order you DON protects YOU; the order you DOFF protects EVERYONE ELSE. Donning moves clean-to-covered (hand hygiene -> gown -> mask/respirator -> eye protection -> gloves). Doffing reverses the contamination logic, removing the dirtiest item first. Gloves are the bookends: last on, first off.

DON (clean-to-covered) vs DOFF (dirtiest first)

DON orderDOFF order
Step 1Hand hygieneGloves
Step 2GownHand hygiene
Step 3Mask / respiratorGoggles / face shield
Step 4Goggles / face shieldGown
Step 5GlovesMask / respirator
FinalEnter roomHand hygiene

DON order

Step 1
Hand hygiene
Step 2
Gown
Step 3
Mask / respirator
Step 4
Goggles / face shield
Step 5
Gloves
Final
Enter room

DOFF order

Step 1
Gloves
Step 2
Hand hygiene
Step 3
Goggles / face shield
Step 4
Gown
Step 5
Mask / respirator
Final
Hand hygiene
Gloves Hallmark
FIRST off; most contaminated; glove-in-glove technique
Hand hygiene
immediately after glove removal
Gown
break ties, roll contaminated surface inward
Goggles or face shield
lift by the back strap, not the front lens
Mask or N95 respirator
LAST off; ties/elastic only, never the front; outside the room
Hand hygiene
again after all PPE removed
Gloves are dirtiest
why they come off first
Touch only insides, backs, ties, straps
the outside of every item is contaminated
Roll gown inward
contains contamination; never fold outward
Mask removed outside the room
protected the airway throughout doffing
Hand hygiene at two points
after gloves AND after all PPE off
Gloves over gown cuffs when donning
continuous barrier at the wrist
Doff at the doorway or anteroom
Hand hygiene required even if gloves intact
contamination occurs during removal
Report Nowescalate immediately
Touching mask front with bare hands
most contaminated surface; direct self-contamination
PPE breach or self-contamination during doffing
perform hand hygiene; follow exposure protocol
Donning error before room entry
stop, remove PPE, restart correct sequence

Clinical Pearl

Gloves first off, mask last off — dirtiest comes off first. On: Gown-Mask-Eyes-Gloves. Off: reverse it. Gloves are the bookends — last on, first off.

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